MeAdipose-derived stem cells (ASCs) have stimulatory effects on dermal papilla (DP) cells to promote hair-growth. For example, ASCs secrete multiple growth factors, such as vascular endothelial growth factor (VEGF) and basic fibroblast growth factors (bFGF), which can increase the
proliferation of DP cells.
Urinary incontinence is a common medical problem, affecting more than 200 million patients in the world. It is much more common in female than in male population. International Consultation on incontinence reported that approximately 49% of all urinary incontinent women suffered from stress urinary incontinence (SUI), 22% from urge incontinence and 29% from mixed incontinence.
ADSC's in Tx of Female Stress Urinary Incontinence March 2017The treatment of mild to moderate osteoarthritis can be a challenging problem for orthopaedic surgeons. As new research and treatment strategies have emerged, stem cell therapy has risen in popularity for the management of degenerative joint conditions. In this article, we describe a stepwise technical approach with tips and pearls to performing adipose-derived stem cell transplantation for degenerative joint disease of the knee.
ADSC Transplant Technique in Tx of DJD Oct 2017Rotator cuff tears are a growing problem within orthopedics as the incidence increases within an aging population, and repair of these tears has been estimated to save society approximately $ 3.4 Billion per year.
PRP-and-Allografts-in-Rotator-Cuff-Repairs-Feb-2018Brown and white adipose tissue is a source of mesenchymal stem cells, specifically adipose-derived stem cells (ADSCs). It is an inexpensive, unlimited reservoir of stem cells. From
300 ml of adipose tissue, 2-3 × 108 ADSCs can be obtained.
Adipose tissue-derived mesenchymal stem cells (AT-MSCs) offer potential as a therapeutic option for chronic discogenic low back pain (LBP) because of their immunomodulatory functions and capacity for cartilage differentiation. The goal of this study was to assess the safety and tolerability of a single intradiscal implantation of combined AT-MSCs and hyaluronic acid (HA) derivative in patients with chronic discogenic LBP.
ADSC's in Chronic Low Back PainMesenchymal stem cells (MSCs) are promising candidates for cell-based therapies. Human platelet
lysate represents an efficient alternative to fetal bovine serum for clinical-scale expansion of MSCs. Different media
used in culture processes should maintain the biological characteristics of MSCs during multiple passages. However,
bone marrow-derived MSCs and adipose tissue-derived MSCs have not yet been directly compared with each other
under human platelet lysate conditions. This study aims to conduct a direct head-to-head comparison of the biological
characteristics of the two types of MSCs under human platelet lysate-supplemented culture conditions for their ability
to be used in regenerative medicine applications.
Mesenchymal stem cells (MSC) represent a promising tool for new clinical concepts
in supporting cellular therapy. Bone marrow (BM) was the first source reported to
contain MSC. However, for clinical use BM may be detrimental due to the highly
invasive donation procedure and the decline in MSC number and differentiation
potential with increasing age. More recently, umbilical cord blood (UCB), attainable
by a less invasive method, was introduced as an alternative source for MSC. Another
promising source is adipose tissue (AT). We compared MSC derived from these
sources regarding morphology, the success rate to isolate MSC, colony-frequency,
expansion potential, multiple differentiation capacity and immune phenotype.
No significant differences concerning the morphology and immuno phenotype of the
MSC derived from these sources were obvious. Differences could be observed
concerning the success rate of isolating MSC, which was 100% for BM and AT, but
only 63% for UCB. The colony-frequency was lowest from UCB whereas it was
highest from AT. However, UCB-MSC could be cultured longest and showed the
highest proliferation capacity, whereas BM-MSC possessed the shortest culture
period and the lowest proliferation capacity. Most strikingly, UCB-MSC showed no
adipogenic differentiation capacity, in contrast to BM- or AT-MSC.
Both UCB and AT are attractive alternatives to BM in isolating MSC: AT as it contains
MSC at highest frequency and UCB as it seems to be expandable to higher numbers.
Various source-derived mesenchymal stem cells (MSCs) have been considered for cell therapeutics in incurable diseases. To characterize MSCs from different sources, we compared human bone marrow (BM), adipose tissue (AT), and umbilical cord blood-derived MSCs (UCB-MSCs) for surface antigen expression, differentiation ability, proliferation capacity, clonality, tolerance for aging, and paracrine activity. Although MSCs from different tissues have similar levels of surface antigen expression, immunosuppressive activity, and differentiation ability, UCB-MSCs had the highest rate of cell proliferation and clonality, and significantly lower expression of p53, p21, and p16, well known markers of senescence. Since paracrine action is the main action of MSCs, we examined the anti-inflammatory activity of each MSC under lipopolysaccharide (LPS)-induced inflammation. Co-culture of UCB-MSCs with LPS-treated rat alveolar macrophage, reduced expression of inflammatory cytokines including interleukin-1α (IL-1α), IL-6, and IL-8 via angiopoietin-1 (Ang-1). Using recombinant Ang-1 as potential soluble paracrine factor or its small interference RNA (siRNA), we found that Ang-1 secretion was responsible for this beneficial effect in part by preventing inflammation. Our results demonstrate that
primitive UCB-MSCs have biological advantages in comparison to adult sources, making
UCB-MSCs a useful model for clinical applications of cell therapy.